pcv2 orf2 gene Search Results


pcv2 orf2 gene  (GenScript corporation)
90
GenScript corporation pcv2 orf2 gene
Pcv2 Orf2 Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvl1392 plasmid containing the pcv2 orf2 gene  (Becton Dickinson)
90
Becton Dickinson pvl1392 plasmid containing the pcv2 orf2 gene
Pvl1392 Plasmid Containing The Pcv2 Orf2 Gene, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dnasis max  (Miraibio Inc)
90
Miraibio Inc dnasis max
Dnasis Max, supplied by Miraibio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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baculogold  (Becton Dickinson)
90
Becton Dickinson baculogold
Baculogold, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgem-t-easy vector  (Promega)
90
Promega pgem-t-easy vector
Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pcv2-specific rpa primers probes  (TwistDx Inc)
90
TwistDx Inc pcv2-specific rpa primers probes
Evaluation of the specificity of <t> PCV2 </t> real-time RPA assay and <t> PCV2 </t> RPA LFD assay.
Pcv2 Specific Rpa Primers Probes, supplied by TwistDx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcv2-specific rpa primers probes/product/TwistDx Inc
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rpa primers and probes  (TwistDx Inc)
90
TwistDx Inc rpa primers and probes
Evaluation of the specificity of <t> PCV2 </t> real-time RPA assay and <t> PCV2 </t> RPA LFD assay.
Rpa Primers And Probes, supplied by TwistDx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxford nanopore minion device  (Oxford Nanopore)
90
Oxford Nanopore oxford nanopore minion device
Evaluation of the specificity of <t> PCV2 </t> real-time RPA assay and <t> PCV2 </t> RPA LFD assay.
Oxford Nanopore Minion Device, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amdv genesig advanced real-time pcr detection kit  (PrimerDesign Inc)
90
PrimerDesign Inc amdv genesig advanced real-time pcr detection kit
Samples taken of each element of biosecurity prior and after visit.
Amdv Genesig Advanced Real Time Pcr Detection Kit, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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one step rt pcr kit  (Qiagen)
99
Qiagen one step rt pcr kit
Comparison among the newly developed triplex <t> SIV, </t> PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.
One Step Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porcilis pesti  (Intervet International GmbH)
90
Intervet International GmbH porcilis pesti
Comparison among the newly developed triplex <t> SIV, </t> PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.
Porcilis Pesti, supplied by Intervet International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prevenile  (Intervet International GmbH)
90
Intervet International GmbH prevenile
Comparison among the newly developed triplex <t> SIV, </t> PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.
Prevenile, supplied by Intervet International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prevenile/product/Intervet International GmbH
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Image Search Results


Evaluation of the specificity of PCV2 real-time RPA assay and PCV2 RPA LFD assay.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: Evaluation of the specificity of PCV2 real-time RPA assay and PCV2 RPA LFD assay.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques:

RPA primers and probes designed in this research.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: RPA primers and probes designed in this research.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Sequencing

Optimal primers and probe combinations of PCV2 real-time RPA assay. The amplification results of nine different combinations of primers with the probe PCV2 RPA Pe are shown.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: Optimal primers and probe combinations of PCV2 real-time RPA assay. The amplification results of nine different combinations of primers with the probe PCV2 RPA Pe are shown.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Amplification

The sensitivity of PCV2 real-time RPA assay. (a) Fluorescence performance via real-time test using a dilution range of pPCV2/RPA. (b) Reproducibility of the PCV2 real-time RPA assay according to eight test runs. (c) Probit regression analysis was done on data from the eight runs of PCV2 real-time RPA assay.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: The sensitivity of PCV2 real-time RPA assay. (a) Fluorescence performance via real-time test using a dilution range of pPCV2/RPA. (b) Reproducibility of the PCV2 real-time RPA assay according to eight test runs. (c) Probit regression analysis was done on data from the eight runs of PCV2 real-time RPA assay.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Fluorescence

Evaluation of the sensitivity of PCV2 RPA LFD assay. (a) In the lateral flow format (PCV2 RPA LFD) the sensitivity was 10 2 copies of the standard DNA. (b) Positive PCV2 RPA LFD reaction products (146 bp) could be detected on a stained agarose gel (3%).

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: Evaluation of the sensitivity of PCV2 RPA LFD assay. (a) In the lateral flow format (PCV2 RPA LFD) the sensitivity was 10 2 copies of the standard DNA. (b) Positive PCV2 RPA LFD reaction products (146 bp) could be detected on a stained agarose gel (3%).

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Staining, Agarose Gel Electrophoresis

(a) Specificity test results of PCV2 RPA LFD assay using total DNA extracted from PCV2 virus and other virus. (b) Positive PCV2 RPA LFD reaction products (146 bp) could be detected on a stained agarose gel (3%).

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: (a) Specificity test results of PCV2 RPA LFD assay using total DNA extracted from PCV2 virus and other virus. (b) Positive PCV2 RPA LFD reaction products (146 bp) could be detected on a stained agarose gel (3%).

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Staining, Agarose Gel Electrophoresis

Comparison of PCV2 real-time RPA assay and PCV2 RPA LFD assay with real-time qPCR assay on clinical samples.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: Comparison of PCV2 real-time RPA assay and PCV2 RPA LFD assay with real-time qPCR assay on clinical samples.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques:

The performance of innuPREP MP basic kit on PCV2 positive samples ( n = 31) was tested by real-time RPA assay, RPA LFD assay, and real-time qPCR assay, respectively.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: The performance of innuPREP MP basic kit on PCV2 positive samples ( n = 31) was tested by real-time RPA assay, RPA LFD assay, and real-time qPCR assay, respectively.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques:

Optimal detection conditions of PCV2 RPA LFD assay. (a)The assay works in a broad range of temperatures. (b) After 10 min of amplification, the test line is visible on the lateral flow dipstick.

Journal: BioMed Research International

Article Title: Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

doi: 10.1155/2017/8403642

Figure Lengend Snippet: Optimal detection conditions of PCV2 RPA LFD assay. (a)The assay works in a broad range of temperatures. (b) After 10 min of amplification, the test line is visible on the lateral flow dipstick.

Article Snippet: After aligning the ORF2 gene consensus sequence of KR559725.1, KR559695.1, KM455975.1, KP768481.1, JN181905.1, JN181902.1, KF850461.1, KC620515.1, KF035059.1, KF951567.1, KF951570.1, KM624031.1, and JQ002672.1, we designed PCV2-specific RPA primers and probes in accordance with TwistDx RPA kits guidelines (Cambridge, United Kingdom).

Techniques: Amplification

Samples taken of each element of biosecurity prior and after visit.

Journal: PLoS ONE

Article Title: Molecular assessment of visitor personal protective equipment contamination with the Aleutian mink disease virus and porcine circovirus-2 in mink and porcine farms

doi: 10.1371/journal.pone.0203144

Figure Lengend Snippet: Samples taken of each element of biosecurity prior and after visit.

Article Snippet: The commercial kits AMDV Genesig Advanced Real-Time PCR Detection Kit (Primerdesign Ltd, Cambridge, UK) and EXOone PCV2 oneMIX (EXOPOL SL, Zaragoza, Spain) were used for amplifying AMDV NS1 and PCV2 orf-2 genes, respectively.

Techniques:

Results of qPCR from personal protective equipments after the visit to positive farms to AMDV and PCV-2.

Journal: PLoS ONE

Article Title: Molecular assessment of visitor personal protective equipment contamination with the Aleutian mink disease virus and porcine circovirus-2 in mink and porcine farms

doi: 10.1371/journal.pone.0203144

Figure Lengend Snippet: Results of qPCR from personal protective equipments after the visit to positive farms to AMDV and PCV-2.

Article Snippet: The commercial kits AMDV Genesig Advanced Real-Time PCR Detection Kit (Primerdesign Ltd, Cambridge, UK) and EXOone PCV2 oneMIX (EXOPOL SL, Zaragoza, Spain) were used for amplifying AMDV NS1 and PCV2 orf-2 genes, respectively.

Techniques: Virus

Results of the qPCR in the environmental surface samples.

Journal: PLoS ONE

Article Title: Molecular assessment of visitor personal protective equipment contamination with the Aleutian mink disease virus and porcine circovirus-2 in mink and porcine farms

doi: 10.1371/journal.pone.0203144

Figure Lengend Snippet: Results of the qPCR in the environmental surface samples.

Article Snippet: The commercial kits AMDV Genesig Advanced Real-Time PCR Detection Kit (Primerdesign Ltd, Cambridge, UK) and EXOone PCV2 oneMIX (EXOPOL SL, Zaragoza, Spain) were used for amplifying AMDV NS1 and PCV2 orf-2 genes, respectively.

Techniques: Virus

Comparison among the newly developed triplex SIV, PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.

Journal: Journal of Virological Methods

Article Title: A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses

doi: 10.1016/j.jviromet.2016.08.005

Figure Lengend Snippet: Comparison among the newly developed triplex SIV, PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.

Article Snippet: The ORF 7 gene of PRRSV-NA and PRRSV-EU, M gene of SIV and ORF2 gene of PCV2 were amplified using QIAGEN one step RT-PCR kit (Qiagen, Valencia, CA, USA) or ExTaq PCR kit (TaKaRa, Mountain View, CA, USA) and cloned into pCR 2.1 vector using TOPO TA cloning kit (Invitrogen/Life Technologies, Grand Island, NY).

Techniques: Comparison, Quantitative RT-PCR